2025-10-14
Mengistu Lemma Lebena, Meseret Girma, Negassi Ameha, Ali Beker, Tesfaheywet Zeryehun, Metages Yirgalem
Experiments were conducted to examine the effect of locally extracted phytase on the performance of broilers with or without phytase supplementation on the blood hematological and serum biochemistry of broiler chickens. A total of 180-day-old Cobb 500 broilers were randomly assigned to four treatment diets in a completely randomized design (CRD), with 15 chicks per replicate and three replications. The experiment used a completely randomized design (CRD), and the birds were fed experimental feed containing phytase at 0, 300, 600, and 1200 FTU/kg for 42 days. There were significant differences (P<0.05) among treatments for Packed cell volume (PCV), Hb, and WBC. However, there were no significant (P>0.05) differences in RBC count, MCV, MCH, or MCHC of broilers fed the diet supplemented with enzymes. Total protein, albumin, and globulin values did not increase significantly (P>0.05) in the supplemented groups compared with the T1 control group throughout the growth period. The calcium concentration ranged from 1-1.49% and 0.75-0.89% in starter and finisher diets, respectively, whereas phosphorus ranged from 0.37-0.52% and 0.28-0.44% in starter and finisher diets, respectively, and it is within the range recommended in the broiler ration. According to the results of this partial budget analysis, T4 (1200 FTU/kg) broiler chickensachieved higher profits than chickens classified as T2, T3, and T1, respectively. Using phytase may be more profitable than feeding control. There was no significant difference in mortality between treatments (P>0.05).
2025-10-14
Riuh Wardhani, Ulil Afidah, Dewi Syamsurya, Lee Jumi, Abera Jabessa Fufa, Fuad Gani, Dirayah Rauf Husain
This study investigated the isolation and characterization of probiotic bacteria from the intestines of genetically unselected Indonesian local chickens ( Gallus gallus domesticus) to improve the quality of broiler chicken meat. Due to their resilience and adaptability, Indonesian local chickens serve as a valuable source of probiotic bacteria. Probiotics were selected using MRSA (de Man, Rogosa, and Sharpe Agar) medium supplemented with 1% CaCO 3 . The probiotic potential of bacterial isolates was assessed based on their resistance to gastric acidity (pH) and bile salts. Characterization included colony morphology, pathogenic inhibition assays using Escherichia coli and Salmonella typhi , and biochemical tests such as MR-VP, TSIA, motility, and catalase. Among the four probiotic bacterial isolates obtained, three were Gram-positive (J2, J3, and J4) and one was Gram-negative (J1). All isolates were rod-shaped (bacilli), catalase-negative, non-motile, and inhibited E. coli and S. typhi . Isolate J2 exhibited the most potent inhibitory activity, with inhibition zones measuring 15.5 mm against S. typhi and 11.5 mm against E. coli . When used as a feed additive, the J2 isolate improved broiler meat quality by maintaining optimal pH, enhancing water-holding capacity, and reducing cholesterol and fat content, although it increased cooking losses. These results indicate that the J2 isolate has significant potential as a probiotic agent in poultry nutrition, with promising implications for improving meat quality and food safety. Future studies should focus on optimizing probiotic formulations and exploring the long-term effects on poultry health and productivity.
2025-10-14
Raden Iis Arifiantini, Khalis Talitha Gusman, Muhammad - Agil, Trinil Susilawati, Syahruddin Said, Harry Cakra Mahendra
In Indonesia, there are 21 frozen semen producers, each with a different stud herd, diluent type, and production target. The present study was conducted to evaluate the performance of these producers. A survey method was employed, utilising a questionnaire (via Google Forms) completed by 21 laboratory personnel from frozen semen producers in Indonesia. The collected data were analysed descriptively and presented as numbers and percentages. The study revealed that producers' performance varied, with the number of laboratory workers ranging from 1 to more than 5, and that men and women (76.19%) were the dominant group. The laboratory personnel's educational backgrounds were predominantly in animal science (54.29%), while the number of bulls owned ranged from less than 10 to more than 200 (90.48% and 9.52%, respectively). The bulls kept were primarily exotic and local cattle (76.19% of the total). The most commonly used diluents were homemade (71.43%), and semen dilution was predominantly performed in a single step. The sperm concentration of fresh semen was measured by 100% of producers using a photometer (SDM or AccuCell); however, internal calibration was performed by 71.43% of producers. Moreover, the evaluation of frozen semen quality focused predominantly on post-thaw motility (100%), with only 61.90% of producers calculating sperm concentration. Furthermore, supplementary assessments, including sperm viability and morphology, were conducted in only 52.38% of cases, with only 4.76% of producers assessing sperm plasma membrane integrity. The proportions of frozen semen samples that exceeded, met, or did not meet the SNI were 80.95%, 14.29%, and 4.76%, respectively.