← Back to Journals

Journal of Seed Science

Publisher:
—
ISSN:
2317-1537
Category:
AGRONOMY
Impact factor:
1.1
Official website ↗Official RSS source ↗

Feed status

2 parsed articles

Last update: Not fetched

Latest articles

Ultrasound-induced dormancy breaking in <i>Arachis pintoi</i> Krapov. &amp; W.C. Greg.: enzymatic and physiological insights

2020-08-09

Baute, Júlia Lima, Casagrande, Daniel Rume, Gontijo, Gabriela Ribeiro, Januario, Jaqueline Pereira, Santos, Heloisa Oliveira dos, Briceño-Pinzón, Ivan David, Pires, Raquel Maria de Oliveira

Baute, Júlia Lima ; Casagrande, Daniel Rume ; Gontijo, Gabriela Ribeiro ; Januario, Jaqueline Pereira ; Santos, Heloisa Oliveira Dos ; Briceño-Pinzón, Ivan David ; Pires, Raquel Maria De Oliveira ; ABSTRACT: Forage peanut is an important crop for animal feed; however, seed dormancy hinders field establishment. This study aimed to identify effective methods to overcome seed dormancy and improve germination. The experiment was conducted in a factorial design with three dormancy-breaking methods (ultrasound, gibberellic acid, and ethephon), three ultrasound exposure times (10, 15, and 20 minutes), and three drying periods (3, 6, and 9 days), in addition to a control. Germination, seedling growth, biomass, and enzymatic activity were evaluated. The experimental design was completely randomized. Data were analyzed using ANOVA, followed by the Scott-Knott test (5%) and Dunnett’s test, and enzymatic profiles were interpreted using zymograms and the ImageJ software. The most effective treatment was ultrasound for 15 minutes, followed by drying the seeds at 50 °C for 9 days, which increased germination to approximately 60%. Higher concentrations of ethephon caused phytotoxicity, while gibberellic acid showed low effectiveness. Enzymatic profiles varied among treatments; the highest alpha-amylase and esterase activities in the ultrasound treatments indicated greater mobilization of seed reserves.

Development of a specific protocol for the tetrazolium test and evaluation of the physiological potential of <i>Cnidoscolus quercifolius</i> Pohl (Euphorbiaceae) seeds

2020-08-09

Silva, Jailton de Jesus, Silva, Jamille Cardeal da, Gomes, Raquel Araujo, Oliveira, Visêldo Ribeiro de, Dantas, Bárbara França

Silva, Jailton De Jesus ; Silva, Jamille Cardeal Da ; Gomes, Raquel Araujo ; Oliveira, Visêldo Ribeiro De ; Dantas, Bárbara França ; ABSTRACT: Rapid tests are essential for assessing the physiological quality of seeds, especially in native Caatinga species that remain little studied. Cnidoscolus quercifolius (Pohl) has ecological and socioeconomic importance but lacks protocols for determining viability. This study aimed to standardize the tetrazolium test (TZT) for evaluating C. quercifolius seeds. Three seed lots were used, from Massaroca (BA), Sento Sé (BA), and Petrolina (PE). The seeds were preconditioned for 16 h at 30 °C on moistened paper and then had the seed coat removed. The experiment followed a completely randomized design in a 7 × 4 factorial arrangement, with seven concentrations of 2,3,5 triphenyl tetrazolium chloride (0.025 to 0.75%) and four immersion times (1 to 4 h), with four replications of 25 seeds. Viability was assessed by the intensity and uniformity of carmine-red staining in the embryo and cotyledons. Low tetrazolium chloride concentrations (≤ 0.025%) underestimated viability, whereas high ones (≥ 0.5%) caused overstaining. Thus, thee range of 0.05 to 0.075% for 3-4 h showed greater diagnostic efficiency, reflecting germination percentages. TZT distinguished viable seeds, with uniform staining and turgid tissues, from non-viable ones. It is concluded that TZT is rapid, reliable, and applicable to programs for conservation, seedling production, and ecological restoration in the Caatinga.