DNA assembly with Sidewinder via 3-way junctions
2026-04-01
Kaihang Wang
Nature Reviews Genetics, Published online: 01 April 2026; doi:10.1038/s41576-026-00955-1 In this Tools of the Trade article, Kaihang Wang describes Sidewinder, a new tool that leverages ‘page number’ barcodes and 3-way junctions to scarlessly assemble DNA with high fidelity, expanding the scope of what DNA writing can achieve.