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2026-03-30
Chiara Lago, Gloria Leva, Marcel Kool, Evelina Miele, Luca Tiberi
Nature Protocols, Published online: 30 March 2026; doi:10.1038/s41596-026-01347-9 This is a protocol for the generation of pediatric patient-derived ependymoma and medulloblastoma tumoroids from surgically resected patient tumor tissues or xenografts, including their use in drug screening and in downstream analyses.
2026-03-24
Runda Xu, Tingting Cong, Junsong Yuan, Xuancheng Chen, Yinqing Li, Xun Lan, Ming Zhu
Nature Protocols, Published online: 24 March 2026; doi:10.1038/s41596-025-01331-9 This step-by-step protocol describes a versatile approach for assessing genome-wide off-target activity of diverse genome editors by tracking replication protein A—a key protein that binds single-stranded DNA intermediates.
2026-03-23
Yinglong Wu, Chaobang Zhang, Junjie Chen, Fang Zeng, Shuizhu Wu, Yanli Zhao
Nature Protocols, Published online: 23 March 2026; doi:10.1038/s41596-026-01338-w This is a protocol for the preparation and characterization of the activatable BTPE-NO2@F127 nanoprobe and its applications in multispectral optoacoustic and near-infrared region II fluorescence dual-mode imaging of liver injury models in mice.
2026-03-20
Fangting Zuo, Ni Su, Xin Xie, Li Jiang, Mengyue Fang, Yuzheng Zhao, Linyong Zhu, Xianjun Chen, Yi Yang
Nature Protocols, Published online: 20 March 2026; doi:10.1038/s41596-026-01343-z This protocol provides guidelines for using fluorescent RNAs, entities consisting of an RNA aptamer bound to its cognate fluorogenic dye, for live imaging of the localization and dynamics of different RNA species in bacteria and mammalian cells.
2026-03-19
Lei Yuan, Ruolan Tang, Shaofang Xie, Zuobao Lin, Pei Cai, Shang Cai
Nature Protocols, Published online: 19 March 2026; doi:10.1038/s41596-026-01333-1 This Protocol generates mouse mammary organoids with the tree-like architecture and cellular heterogeneity of native tissue, which recapitulate mammary physiology across the estrous cycle, pregnancy, lactation and involution.