2020-08-09
Rachmawati, Yuanita Lely, Agnizarridlo, Tubagus, Fuadiyah, Diena, Aziz, Shaffiratul, Arifin, Zainal, Alvianti, Kurnia Putri
Rachmawati, Yuanita Lely ; Agnizarridlo, Tubagus ; Fuadiyah, Diena ; Aziz, Shaffiratul ; Arifin, Zainal ; Alvianti, Kurnia Putri ; ABSTRACT Objective: To assess stress, anxiety, and depression among preclinical and clinical dental students during online learning, as well as the factors that contributed to these conditions. Material and Methods: A cross-sectional study was conducted in late 2021 involving 580 dental students from Indonesian dental faculties. Data were collected via an online questionnaire distributed via social media, using a snowball sampling approach. The questionnaire included informed consent, sociodemographic data, behavioral factors, lecture activities, and the Depression Anxiety Stress Scale (DASS-21). Data analysis was performed using Chi-square and logistic regression. Results: The prevalence of anxiety, stress, and depression among the total 607 students enrolled was 70.8%, 63.1%, and 51.6%, respectively, with preclinical students showing higher levels. Regular exercise was associated with a 37% reduction in stress, while attending online shortly lowered the likelihood of anxiety by 56%. Conversely, having family or friends diagnosed with COVID-19 increased the risk of depression by 1.53 and 1.88 times, respectively. Conclusion: Stress was related to lack of exercise and insufficient sleep, anxiety to higher online learning frequency, and depression was reduced by extracurricular activities but worsened by COVID-19 cases among family or friends.
2020-08-09
Massola Filho, Francisco, Monteiro, Ulysses de Toledo, Ferreira, Mateus Gambi de Medeiros, Franco, Amanda Gonçalves, Kuga, Milton Carlos, Campos, Edson Alves de
Massola Filho, Francisco ; Monteiro, Ulysses De Toledo ; Ferreira, Mateus Gambi De Medeiros ; Franco, Amanda Gonçalves ; Kuga, Milton Carlos ; Campos, Edson Alves De ; ABSTRACT Objective: To characterize the microscopic structure and surface properties of commercially available 3D-printed resin composites using Scanning Electron Microscopy (SEM) to assess their clinical applicability and durability. Material and Methods: Four different 3D-printed resin composites were analyzed. Specimens were fabricated under controlled conditions, followed by standardized cleaning, post-curing, and polishing procedures. SEM imaging was performed at various magnifications to evaluate filler distribution, particle morphology, and surface integrity. Results: The analysis revealed significant differences among the tested resins. Varseo Smile Crown Plus (Bego) exhibited the most homogeneous microstructure, with a high filler content, nanoscale particles, and strong polymer-filler integration, suggesting superior mechanical properties. Biocrown and Nanolabs resins showed intermediate levels of filler content and particle distribution. At the same time, Printax AA Temp had the lowest inorganic content and the highest incidence of structural defects, including layer discontinuities and cracks. Conclusion: The structural and surface characteristics of 3D-printed resin composites vary significantly among different commercial brands. Varseo Smile Crown Plus demonstrated the most promising properties for clinical applications, while other materials showed limitations related to filler distribution and structural integrity. Further studies on mechanical performance and long-term stability are needed to optimize the application of these materials in definitive restorations.
2020-08-09
D’Assunção, Nathália Cunha Lima, Lima, Andrea Marcia da Cunha, Gomes, Arthur Barbosa, Sousa, Fátima Regina Nunes de
D’assunção, Nathália Cunha Lima ; Lima, Andrea Marcia Da Cunha ; Gomes, Arthur Barbosa ; Sousa, Fátima Regina Nunes De ; ABSTRACT Objective: To estimate the prevalence of facial paralysis in patients with malignant parotid tumors who underwent parotidectomy. Material and Methods: The method employed was a systematic review, with a search conducted in the following databases: PubMed, Embase, Scopus, Web of Science, and LILACS. Grey literature from Google Scholar and ProQuest Dissertations and Theses was also used. The included studies were observational, performed with patients with malignant parotid tumors, diagnosed from the histopathological examination of the gland, with permanent or transient facial paralysis, verified by clinical examination, as a result of surgical treatment for the neoplasm. Results: Among the 2156 studies identified, 16 were selected for this systematic review. The prevalence of facial paralysis after parotidectomy due to malignancy varied according to the type of facial paralysis, the size of the tumor, the kind of parotidectomy performed, the presence of preoperative facial paralysis, and the need for adjuvant radiotherapy. Conclusion: The prevalence of facial paralysis after parotidectomy due to malignancy ranged from 9.09% to 100% among the studies.
2020-08-09
Nunes, Natália Novais Vasconcelos, Oliveira, Rafaela Silva, Coutinho, Luciene Souza, Odilon, Natália Nascimento, Campos, Elisângela de Jesus
Nunes, Natália Novais Vasconcelos ; Oliveira, Rafaela Silva ; Coutinho, Luciene Souza ; Odilon, Natália Nascimento ; Campos, Elisângela De Jesus ; ABSTRACT Objective: To evaluate the effect of dentifrices labeled as vegan on the mass and surface roughness of a nanoparticulate composite resin subjected to simulated toothbrushing, and to analyze the labeling of these products. Material and Methods: Sixty composite resin specimens were divided into six groups (n=10): one control group using a conventional dentifrice (CG) and five test groups using dentifrices labeled as vegan (TG1, TG2, TG3, TG4, and TG5). Mass and surface roughness were measured before and after simulated brushing equivalent to six months (25,000 cycles) and twelve months (50,000 cycles) of use. The labeling analysis assessed the composition of the dentifrices and their compliance with Brazilian Health Regulatory Agency regulations. Results: Mass analysis showed no significant differences between groups at either time point. Regarding surface roughness, the control group showed no significant change (p>0.05) after 6 and 12 months of brushing. However, the test groups exhibited variable responses. After twelve months, TG4 exhibited significantly greater roughness compared to CG, TG1, and TG2 (p<0.05), while TG5 showed greater roughness than CG and TG1 (p<0.05). Visual indicators of veganism were present on the packaging of the test dentifrices, although no certification seals were found. Conclusion: The labeling analysis revealed that vegan dentifrices are not currently regulated by national or international agencies, raising concerns about their reliability and safety, as well as the absence of agents with proven anticaries efficacy. Additionally, while brushing with vegan-labeled dentifrices did not significantly increase the roughness or reduce the mass of the composite resin, their effects after twelve months of simulated brushing were comparable to those of conventional dentifrices.
2020-08-09
Reinaldo, Manuel Carlos Gomes, Carvalho, Isabella Fernandes, Silva, Paulo Goberlânio de Barros, Cavalcante, Daniel de Sá, Pereira Filho, Cláudio Roberto Tavares, Alencar, Phillipe Nogueira Barbosa
Reinaldo, Manuel Carlos Gomes ; Carvalho, Isabella Fernandes ; Silva, Paulo Goberlânio De Barros ; Cavalcante, Daniel De Sá ; Pereira Filho, Cláudio Roberto Tavares ; Alencar, Phillipe Nogueira Barbosa ; ABSTRACT Objective: To describe digital intraoral scanning in five children with microcephaly caused by the Zika virus, detailing occlusal and dental characteristics using digital models. Material and Methods: An observational study assessed the dental occlusal profile of five 4-year-old children with Congenital Zika Virus Syndrome (CZS) using digital oral scanning. The children were treated at the Unichristus School of Dentistry Clinic. The Panda 2 intraoral scanner was utilized. A mouth opener made from wooden sticks, adhesive tape, and gauze was used individually for each patient, thereby minimizing the risk of trauma. The upper arches were scanned first, followed by the lower arches, and finally the arches in occlusion. Results: The 3D digital models revealed the relationship between the distal faces of the deciduous second molars, Baume's arch type, tooth eruption chronology, anterior open bite, posterior crossbite, overbite, overjet, and deviated midline. Conclusion: Intraoral scanning in children with congenital Zika virus microcephaly proved to be a viable alternative for producing reliable digital study models, offering more comfort and safety to these patients and replacing conventional molding techniques.
2020-08-09
Melo, Keily da Silva, Melo, Luciana Aleixo de, Lima, Cássia Cunha de, Mendonça, Leilane de Sousa, Aranha, Elenn Suzany Pereira, Vasconcellos, Marne Carvalho de, Bandeira, Maria Fulgência Costa Lima, Toda, Carina, Conde, Nikeila Chacon de Oliveira
Melo, Keily Da Silva ; Melo, Luciana Aleixo De ; Lima, Cássia Cunha De ; Mendonça, Leilane De Sousa ; Aranha, Elenn Suzany Pereira ; Vasconcellos, Marne Carvalho De ; Bandeira, Maria Fulgência Costa Lima ; Toda, Carina ; Conde, Nikeila Chacon De Oliveira ; ABSTRACT Objective: To evaluate the cytotoxic and genotoxic effects of a phytotherapeutic ointment containing 2% L. ferrea L extract. Material and Methods: Libidibia ferrea L. barks were used as raw material. The extract was prepared by decoction under reflux in a hydroalcoholic solution. The extract concentration in the orabase formulation was determined based on the minimum inhibitory concentration. Biocompatibility was assessed by cell culture tests from MRC5 human fibroblasts. The Alamar Blue test evaluated cytotoxicity; the Trypan Blue Solution trial evaluated cell viability. The comet assay on alkaline and neutral pH assessed genotoxicity. Test results were tabulated and analyzed through descriptive and inferential statistics, with ANOVA Test (p≤ 0.05). Results: No cytotoxic activity or morphological induction of cell death for the test formulation. Regarding genotoxicity, the Juca formulation could not induce DNA damage in MRC-5 at 25 and 50 μg/mL concentrations through the comet assay at neutral or alkaline pH. Conclusion: The tested formulation is biocompatible, and no cytotoxic or genotoxic effects were observed under test conditions, demonstrating viability for future in vivo tests.
2020-08-09
Michelin, Maria Eduarda Santos, Barros, Antonia Patricia Oliveira, Gelio, Mariana Bena, Costa, Joatan Lucas de Sousa Gomes, Kuga, Milton Carlos
Michelin, Maria Eduarda Santos ; Barros, Antonia Patricia Oliveira ; Gelio, Mariana Bena ; Costa, Joatan Lucas De Sousa Gomes ; Kuga, Milton Carlos ; ABSTRACT Objective: To evaluate the influence of two antioxidants on the fracture resistance of bleached enamel. It also assessed their impact on color change and surface microhardness. Material and Methods: Forty-eight bovine enamel samples underwent three bleaching sessions and were randomly divided into three groups (n=16): Control – No antioxidant application; Sodium Ascorbate – Application of sodium ascorbate for 30 min; DL-alpha-lipoic Acid – Similar to Sodium Ascorbate, but the antioxidant used was DL-alpha-lipoic Acid. Then, color was evaluated with a spectrophotometer, and microhardness was measured using the Knoop test, before the start of the bleaching treatment and immediately after the final application of the bleaching agent and the antioxidant. Afterward, the specimens were restored with composite resin. After seven days, the fracture resistance test was performed using an electromechanical testing machine. Fracture resistance and color evaluation data were subjected to one-way ANOVA, followed by Tukey’s post-hoc test. Microhardness was assessed using Kruskal-Wallis and Dunn tests (p<0.05). Results: Sodium ascorbate provided the highest fracture resistance compared to DL-alpha-lipoic acid and the control group after bleaching (p<0.05). The antioxidant agents did not interfere with the whitening effect of 35% hydrogen peroxide (p>0.05). Conclusion: Using sodium ascorbate reduced the deleterious effects on enamel microhardness and fracture resistance.
2020-08-09
Kalczuk, Liana, Cortelli, Sheila Cavalca, Aquino, Davi Romeiro, Bonates, Fabrizia Henriques, Neves, Maria Valéria de Campos, Perrenoud, Priscila Bernardo, Silva Sobrinho, Argemiro Soares da, Matieli, José Elias
Kalczuk, Liana ; Cortelli, Sheila Cavalca ; Aquino, Davi Romeiro ; Bonates, Fabrizia Henriques ; Neves, Maria Valéria De Campos ; Perrenoud, Priscila Bernardo ; Silva Sobrinho, Argemiro Soares Da ; Matieli, José Elias ; ABSTRACT Objective: To observe the impact of military training on the oral health of Brazilian Air Force (FAB) recruits in the first phase and to assess the efficacy of ozonated sunflower oil (Oleozon) in improving the periodontal indices of FAB recruits in the second phase. Material and Methods: In the observational phase, PPD (periodontal probing depth), CAL (clinical attachment level), PI (plaque index), GI (gingival index), and DMFT (decayed, missed, and filled teeth) values were compared before and at the end of military training. In the interventional phase, a randomized, double-anonymized, placebo-controlled study was conducted to evaluate whether the daily use of oleozon added to toothpaste would improve periodontal indices. Results: At the end of their military training, recruits exhibited a significant worsening in periodontal disease indicators, resulting in increased dental costs for FAB and increased duty absence. In the second phase, a substantial reduction in PPD and GI (p<0.004 and p<0.001) was observed in the oleozon group. Conclusion: Oleozon, in association with toothpaste brushing, was superior to brushing alone in improving the periodontal health. Despite the need for further studies, it is proposed that oleozon should be included in the oral hygiene protocol for Brazilian Air Force recruits, thereby reducing periodontal risk and dental costs.
2020-08-09
Mansour, Nirvana Khalaf, Ballal, Nidambur Vasudev
Mansour, Nirvana Khalaf ; Ballal, Nidambur Vasudev ; ABSTRACT Objective: To investigate the radiographic periapical healing after root canal treatment using a novel 1-Hydroxyethylidene-1,1-Diphosphonic acid (Dual Rinse HEDP) irrigant in necrotic teeth with an apical periodontitis. Material and Methods: In this clinical study, 65 patients with necrotic molars, premolars, and anterior teeth requiring root canal treatment were enrolled. Before the commencement of the study, all study participants signed informed consent forms individually. Teeth were mechanically prepared with E3 Azure rotary files, irrigated with Dual Rinse HEDP irrigant, and then obturated with a single-cone technique and bio-ceramic sealer. After one year, 50 patients (15 dropped out) were evaluated radiographically for periapical healing using the Periapical Index (PAI) scores. Fisher's Exact test and Friedman's test were used. The significance level was set at 5%. Results: After one year, the PAI scores of all patients decreased significantly (p < 0.001). Regarding pain, there was a statistically significant decrease in the prevalence of pain after one year of follow-up amongst the different groups of teeth (anteriors, premolars, molars) (p < 0.001). Conclusion: Dual Rinse HEDP, in conjunction with sodium hypochlorite, can be used as an effective irrigant to promote periapical healing.
2020-08-09
Ribeiro, Adyelle Dantas, Silva, Damião Romão Dias da, Costa, Mariana Mélani Alexandrino, Marques, Maria Helena Vieira Pereira, Medeiros, Maria Luíza Vieira Pereira de, Tavares, Matheus de Medeiros, Brasil, Arthur Willian de Lima, Freire, Waldênia Pereira, Gomes, Daliana Queiroga de Castro, Figueiredo, Robéria Lucia de Queiroz, Pereira, Jozinete Vieira
Ribeiro, Adyelle Dantas ; Silva, Damião Romão Dias Da ; Costa, Mariana Mélani Alexandrino ; Marques, Maria Helena Vieira Pereira ; Medeiros, Maria Luíza Vieira Pereira De ; Tavares, Matheus De Medeiros ; Brasil, Arthur Willian De Lima ; Freire, Waldênia Pereira ; Gomes, Daliana Queiroga De Castro ; Figueiredo, Robéria Lucia De Queiroz ; Pereira, Jozinete Vieira ; ABSTRACT Objective: To gather epidemiological data on AIDS in Brazil over more than four decades in the population aged 0 to 14 years. Material and Methods: This is a descriptive cross-sectional study. The population corresponded to individuals with AIDS aged 0 to 14 years, with data registered in the Notifiable Diseases Information System, collected through the DATASUS website, reported in Brazil from the first case in July 1982 to 2022. Using descriptive statistics based on the calculation, it was found that there was an association using the Chi-Square test. Furthermore, the Prais-Winsten linear analysis model was used for trend analysis. Results: A total of 28,007 cases were reported. An increase in HIV cases was observed until the beginning of the 2000s. Regarding races/skin color, the majority of affected individuals belonged to the white race (16.75%), second only to those who did not report this characteristic (64.5%). The highest incidence rate was found in the South region, with 475 new cases of AIDS per 100.000 live births. Conclusion: The annual variation rate showed a stationary trend, with a gradual decrease from 2000 to 2022. There was a significant percentage of notifications in which the race/color item was ignored. The most affected age group was 1 to 4 years old.
2020-08-09
Ahmad, Wan Muhamad Amir W, Adnan, Mohamad Nasarudin, Ghazali, Farah Muna Mohamad, Aleng, Nor Azlida, Halim, Nurfadhlina Abdul, Ibrahim, Mohamad Shafiq Mohd
Ahmad, Wan Muhamad Amir W ; Adnan, Mohamad Nasarudin ; Ghazali, Farah Muna Mohamad ; Aleng, Nor Azlida ; Halim, Nurfadhlina Abdul ; Ibrahim, Mohamad Shafiq Mohd ; ABSTRACT Objective: To develop and validate a predictive model for oral squamous cell carcinoma (OSCC) survival outcomes, assessing the impact of key risk factors on survival probability. Material and Methods: A retrospective analysis was performed using a Multilayer Feedforward Neural Network (MLFFNN) in R. The outcome variable was survival status (live or dead), while predictors included smoking status, age, betel quid use, alcohol consumption, and sex. Data were normalized and partitioned into training, testing, and validation sets using bootstrap resampling. The MLFFNN model was optimized with hidden layers and a logistic activation function to enhance predictive performance. Results: Smoking status was the most influential predictor of OSCC survival (24%), followed by age (16.91%), betel quid use (15.37%), sex (10.21%), and alcohol consumption (8.57%). Performance metrics included a Mean Absolute Error of 0.2628, Root Mean Squared Error of 0.3466, and validation accuracy of 73.715%. Conclusion: This study highlights the potential of neural network-based models in predicting OSCC survival outcomes. Smoking emerged as the dominant risk factor, providing critical insights for data-driven clinical management strategies.
2020-08-09
Paiva, Amanda Luisa Rodrigues de, Aragão, Maria Gerusa Brito, Ricomini-Filho, Antônio Pedro, Tabchoury, Cinthia Pereira Machado, Aires, Carolina Patrícia, Cury, Jaime Aparecido
Paiva, Amanda Luisa Rodrigues De ; Aragão, Maria Gerusa Brito ; Ricomini-Filho, Antônio Pedro ; Tabchoury, Cinthia Pereira Machado ; Aires, Carolina Patrícia ; Cury, Jaime Aparecido ; ABSTRACT Objective: To evaluate the fluoride release from Colgate Zero Kids® dentifrice compared to two children’s dentifrices, Tandy® and Sorriso Kids®. Material and Methods: All products, from the same manufacturer, had identical fluoride formulations (NaF/silica; 1,100 ppm F) but differed in rheological properties. A 4-g sample of each dentifrice (4.4 mg F) was agitated in ultrapure water and centrifuged; the supernatant was collected for fluoride analysis using a fluoride ion-selective electrode, calibrated with standards (0.25-16.0 µg F/mL) in 50% TISAB II. Fluoride release was expressed as a percentage of total fluoride, and data (n=6) were statistically analyzed using ANOVA and Tukey’s test (α = 5%). Results: Mean fluoride release was: Tandy® (71.4 ± 3.2%), Sorriso Kids® (70.3 ± 2.1%), and Colgate Zero Kids® (37.7 ± 9.7%), with Colgate differing significantly (p < 0.05). To investigate the lower fluoride release from Colgate Zero Kids®, the flow and viscosity curves of the toothpastes were obtained using a Brookfield R/S Plus rheometer. Colgate Zero Kids® showed significantly higher thixotropy compared to the other dentifrices. Conclusion: The results suggest that the reduced fluoride availability in Colgate Zero Kids® may be attributed to its higher viscosity, potentially limiting fluoride ion diffusion. These findings highlight the importance of considering not only fluoride content but also physicochemical properties such as viscosity in dentifrice formulations for effective fluoride bioavailability.
2020-08-09
Ghasemi, Hadi, Anjomrouz, Fazel, Heydari, Gholamreza
Ghasemi, Hadi ; Anjomrouz, Fazel ; Heydari, Gholamreza ; ABSTRACT Objective: To investigate the impact of an educational intervention on the knowledge, attitude, and skills of dental students regarding smoking cessation. Material and Methods: The participants were dental students in their 7th (Intervention group) and 8th semester (Control group) between February and July 2022. The intervention group received an educational package consisting of a lecture, a podcast, a seminar, and role-playing on smoking cessation, while the control group only received a lecture. The students' knowledge, attitudes, and skills were assessed using a questionnaire. The questionnaire included statements regarding the importance of learning about smoking cessation, the level of students' knowledge of and attitudes towards treating tobacco addiction, and skills related to quitting smoking. Moreover, students' knowledge about the physiology of addiction, non-nicotine drugs for smoking cessation, and electronic cigarettes was evaluated. Results: In total, information from 55 students was used. In the intervention group, an increase was found in the willingness to advise patients from 56% to 92% (p=0.003), the mean score of knowledge regarding electronic cigarettes (3.11±1.65 to 4.19±1.33, p=0.001), agreement with "I can get information about the patient's smoking" from 22% to 90%, "I am able to advise for quitting smoking" from 38% to 81%, "I can provide recommendations for smoking cessation" from 22% to 58%, and "In my opinion, smoking addiction is a chronic disease" from 56% to 77%. Conclusion: Training dental students in smoking cessation counseling can enhance their understanding and motivation to assist patients in quitting smoking.
2020-08-09
Almondes, Camila Maria Simas, Silva, Fábio Mesquita da, Souza, Luana Carneiro Diniz, Carvalho, Alicia Valeria dos Santos Zaranza de, Rodrigues, Vandilson Pinheiro, Lopes, Fernanda Ferreira
Almondes, Camila Maria Simas ; Silva, Fábio Mesquita Da ; Souza, Luana Carneiro Diniz ; Carvalho, Alicia Valeria Dos Santos Zaranza De ; Rodrigues, Vandilson Pinheiro ; Lopes, Fernanda Ferreira ; ABSTRACT Objective: To analyze the oral biofilm and tracheal aspirate of children on invasive mechanical ventilation to observe similarities in the microbiota. Material and Methods: This study consisted of a retrospective descriptive analysis carried out in 35 children admitted to the Pediatric Intensive Care Unit of the University Hospital of the Federal University of Maranhão from March to December 2019. The lingual biofilm and tracheal aspirate were collected, and both samples were sent for culture examination. Results: In 34.3% of participants, the same bacteria were present in both the lingual biofilm and the tracheal aspirate, with Stenotrophomonas maltophilia and Acinetobacter baumannii being the most frequent. The frequency of detection of the same species at both sites was higher among patients who died compared to those discharged from the hospital (54.6% versus 25%, p = 0.087). There was a statistically higher frequency of deaths in patients aged up to 24 months (52.9% versus 11.1%, p = 0.01). Conclusion: There was a high similarity in the microorganisms found at the two sites studied in patients up to 24 months of age. Thus, it is noted that the oral cavity of hospitalized children on invasive mechanical ventilation serves as a reservoir for respiratory pathogens.
2020-08-09
Mendes, Rodrigo Costa, Pedrosa, João Pedro Costa, Rodrigues, Vandilson Pinheiro, Cantanhede, Luana Martins, Neves, Pierre Adriano Moreno
Mendes, Rodrigo Costa ; Pedrosa, João Pedro Costa ; Rodrigues, Vandilson Pinheiro ; Cantanhede, Luana Martins ; Neves, Pierre Adriano Moreno ; ABSTRACT Objective: To summarize data on the prevalence of Molar Incisor Hypomineralization (MIH) in Brazilian children and adolescents and to evaluate its frequency and geographic distribution. Material and Methods: A systematic review was conducted using the following databases: PubMed, Scopus, Latin American Literature in Health Sciences (LILACS), EMBASE, Scientific Electronic Library Online (SciELO), Virtual Health Library (BIREME), Cochrane Library, and Web of Science, covering studies published from 2000 to 2023. The inclusion criteria were studies assessing the prevalence of MIH in Brazilian children and adolescents, published in English, Portuguese, or Spanish, with samples of Brazilians aged 5 to 15 years. Two reviewers conducted the study selection under the PRISMA and MOOSE guidelines. The risk of bias was assessed using the JBI Critical Appraisal Tool for Analytical Cross-Sectional Studies. A total of 12 studies met the inclusion criteria, with a combined sample of 8,312 participants. A meta-analysis was performed to determine the pooled prevalence of MIH using random-effects models. Results: The pooled prevalence of MIH in the Brazilian population is 15.9% (95% CI: 11.3-20.5). The Southeast region has the highest prevalence, at 20.2%, followed by the Northeast at 16.7%, the South at 13%, and the Midwest at 11.5%. Conclusion: These findings suggest that the prevalence of MIH in Brazil is comparable to the global estimate, albeit with regional variations.
2020-08-09
Toprani, Nikita, Acharya, Shashi Rashmi, Prakash, Peralam Yegneswaran, Mayya, Arun, Chatra, Akshatha, Mayya, Shreemathi S., Vimala, N.
Toprani, Nikita ; Acharya, Shashi Rashmi ; Prakash, Peralam Yegneswaran ; Mayya, Arun ; Chatra, Akshatha ; Mayya, Shreemathi S. ; Vimala, N. ; ABSTRACT Objective: To evaluate the antifungal effect of 25% Tea tree oil and compare it to 2% Chlorhexidine and Nystatin against Candida albicans at both the 4th day and 7th day of inoculation to explore potential alternative treatment options for fungal infections in endodontic therapy. Material and Methods: 82 single-canal human mandibular premolar teeth were cleaned, prepared, and inoculated with a C. albicans suspension. After incubating for 4 and 7 days, the teeth were randomly assigned to receive one of four treatments: Nystatin, Chlorhexidine (CHX), Tea tree oil (TTO), or Dimethyl Sulfoxide (DMSO). After treatment, samples were rinsed and dried. Dentine shavings were collected and incubated to observe the growth of C. albicans. The antifungal activity was measured by the number of Candida colony-forming units. Two-way ANOVA followed by post-hoc Tukey's test was applied to compare the CFU growth. Results: TTO exhibited comparable antifungal activity to CHX (p=0.976) and superior antifungal activity compared to Nystatin (p<0.001). Conclusion: 25% Tea tree oil shows antifungal efficacy against C. albicans, comparable to 2% Chlorhexidine, suggesting it could be a viable alternative for endodontic treatments. However, Nystatin demonstrated only limited antifungal effectiveness.
2020-08-09
D’Assunção, Verônica Cabral dos Santos Cunha, D’Assunção, Fábio Luiz Cunha, Xavier, Áthina de Melo, Nascimento, Elizabeth Denize Izidoro do, Silva, Juan Ramon Salazar, Almeida, Leopoldina de Fátima Dantas de
D’assunção, Verônica Cabral Dos Santos Cunha ; D’assunção, Fábio Luiz Cunha ; Xavier, Áthina De Melo ; Nascimento, Elizabeth Denize Izidoro Do ; Silva, Juan Ramon Salazar ; Almeida, Leopoldina De Fátima Dantas De ; ABSTRACT Objective: To evaluate the risk of percutaneous injuries (PCIs) that novice predoctoral operators undergo due to non-compliance with biosafety standards in the Endodontics clinic. Material and Methods: This is a prospective longitudinal observational study, developed for two years, which included 120 dental students from the Endodontic Clinic of Dentistry course. The following circumstances were analyzed: burs left in the handpiece after use; bare needles lying on the instrument tray or being recapped with two hands; cannulas left in the suction; Gates-Glidden drills left in the micromotor; and biosafety violations involving endodontic files. Descriptive and inferential statistical analysis was performed using Chi-square test (α=5%). Results: The sample consisted of 75% women (90). Ninety-nine students (82.50%) were involved in situations that could cause percutaneous injuries. Despite the risk, no accidents occurred during the study period. In total, 364 situations involving risk of percutaneous injuries were observed. In this study, 209 (57.4%) cases of burs left in the handpiece after use were observed, 60 (16.5%) cases of aspiration cannulas left in the suction, 52 (14.3%) cases of bare needles on the instrument tray or being recapped with two hands, 26 (7.1%) cases of Gates-Glidden drills left in the micromotor socket and 17 (4.7%) cases of biosafety violations involving endodontic files. A significant difference (p<0.05) was observed between the 5 types of risk situations. Conclusion: There was a high percentage of the risk of percutaneous injuries in novice predoctoral operators. Burs left in the handpiece after use were the most encountered risk situation, and most novice predoctoral operators in the Endodontic Clinic disrespected biosafety standards.
2020-08-09
Marafiga, Fernanda de Andrade, Souza, Matheus Albino, Dogenski, Letícia Copatti, Dallepiane, Felipe Gomes, Corazza, Pedro Henrique, Benetti, Paula, Bello, Yuri Dal, Bervian, Juliane, Trentin, Micheline Sandini, Cecchin, Doglas, Carli, João Paulo De
Marafiga, Fernanda De Andrade ; Souza, Matheus Albino ; Dogenski, Letícia Copatti ; Dallepiane, Felipe Gomes ; Corazza, Pedro Henrique ; Benetti, Paula ; Bello, Yuri Dal ; Bervian, Juliane ; Trentin, Micheline Sandini ; Cecchin, Doglas ; Carli, João Paulo De ; ABSTRACT Objective: To evaluate the mechanical properties of dentin under the effect of grape seed extract (GSE) as intracanal medication (ICM). Material and Methods: Ninety-six single-rooted bovine teeth were divided into groups, as follows: control group (Ctrl), without any dentin treatment; groups treated with Ca(OH)2 (Ca7, Ca15 and Ca30); and GSE (GSE7, GSE15 and GSE30), with direct contact between dentin and ICM for different durations of 7, 15 and 30 days. The samples underwent tests of maximum fracture load (FL), flexural strength (σf), cohesive strength (σc), and elastic modulus (E) of the remaining root. For statistical analysis, one-way ANOVA was used, followed by Dunnett's test to verify whether ICMs interfere with dentin properties. Two-way ANOVA (ICM and application time) and Tukey's test were also used, considering the groups treated with different ICM at different intervals. All analyses were performed at p ≤ 0.050. Results: GSE had a less pronounced effect on dentin's mechanical properties compared to Ca(OH)2. GSE showed less interference with FL (p=0.000), σf (p=0.002), and σc (p=0.000). It was observed that the duration of exposure to ICM had a negative impact on FL (p=0.002) and σc (p=0.031). ICM (p=0.080) and the application time (p=0.985) did not significantly affect E. Moreover, the application time of ICM did not have a significant impact on σf (p=0.169). Conclusion: Grape seed extract did not affect the mechanical properties of bovine dentin when used as intracanal medication.
2020-08-09
Shiow, Yee Yng, Liszen, Tang, Ahmad, Wan Muhamad Amir W, Adnan, Mohamad Nasarudin, Ghazali, Farah Muna Mohamad
Shiow, Yee Yng ; Liszen, Tang ; Ahmad, Wan Muhamad Amir W ; Adnan, Mohamad Nasarudin ; Ghazali, Farah Muna Mohamad ; ABSTRACT Objective: To present the TL Sharps Bin, a newly developed device designed to enhance safety and functionality in healthcare settings, and to evaluate its performance and usability among healthcare professionals. Material and Methods: A total of 82 participants, comprising 40 dental surgeons and 42 medical doctors, were selected using random sampling. Following a demonstration and hands-on session on the proper use of the TL Sharps Bin, participants completed a structured questionnaire consisting of 19 items. The questionnaire assessed the bin’s performance across two key domains: visibility and accommodation. To analyze the relationship between categorical variables, chi-square tests were applied, allowing for the assessment of associations between participant characteristics and their responses to the questionnaire. Results: The TL Sharps Bin was rated highly in all three dimensions, with safety receiving a mean score of 4.6, usability 4.5, and efficiency 4.7 on a 5-point Likert scale. Participants highlighted the bin’s superior puncture resistance, ease of use, and accessibility in busy clinical settings. Statistical analysis confirmed that the TL Sharps Bin significantly outperformed traditional containers in terms of both user satisfaction and adherence to safety protocols (p<0.05). Conclusion: The TL Sharps Bin demonstrated exceptional performance in clinical settings, meeting and exceeding the safety guidelines set by the World Health Organization and the Occupational Safety and Health Administration. Its adoption is strongly recommended to improve sharps disposal practices and enhance occupational safety for healthcare workers. Further studies could explore its long-term impact on reducing the incidence of sharps-related injuries and infections.
2020-08-09
Barnabé, Luan Éverton Galdino, Fascineli, Maria Luiza, Nóbrega, Vivyanne Falcão Silva da
Barnabé, Luan Éverton Galdino ; Fascineli, Maria Luiza ; Nóbrega, Vivyanne Falcão Silva Da ; ABSTRACT Objective: To evaluate factors associated with the clinical outcomes of individuals with pharyngeal teratoma/epignathus. Material and Methods: An integrative and systematic literature review was conducted using the PubMed, Scopus, Web of Science, and BVS databases, with the descriptors "Pharynx" and "Teratoma" or "Epignathus," selecting case reports and series published in English between 2014 and 2024. The search was performed in May 2024. Inclusion criteria followed the WHO (2022) recommendations, selecting cases of tumors with pharyngeal implantation that histologically presented the three embryonic germ layers. Statistical analysis was performed descriptively. Results: 41 studies were selected, comprising 42 cases of epignathus. Cases were categorized based on clinical outcomes as survival (25 cases) or death (17 cases). The mean maternal age was 28.47 ± 6.30 years, with polyhydramnios being the most reported gestational complication (40.7%). There was a slight predominance of female cases (1.5:1). The oral portion of the pharynx was the most common anatomical site of tumor implantation (73.8%). The EXIT procedure was employed to prevent respiratory distress and improve neonatal survival (23.8%). Partial surgical excision of the tumors was directly associated with lesion recurrence (26.2%). Conclusion: Identification of the lesion at ≤20 weeks of gestation, rapid tumor growth, predominance of immature tissues, intracranial involvement, and the presence of severe developmental abnormalities in the nervous and cardiocirculatory systems negatively impact the outcomes of epignathus cases.
2020-08-09
Nóbrega, Johnys Berton Medeiros da, Gusmão, Carolina Lucena Veloso, Laureano, Isla Camilla Carvalho, Santiago, Bianca Marques
Nóbrega, Johnys Berton Medeiros Da ; Gusmão, Carolina Lucena Veloso ; Laureano, Isla Camilla Carvalho ; Santiago, Bianca Marques ; ABSTRACT Objective: To investigate ChatGPT®’s knowledge of Brazilian dental ethics and legislation. Material and Methods: A cross-sectional study analyzed ChatGPT® 3.5’s responses to 281 dichotomous questions (“yes” or “no”) based on Federal Council of Dentistry (CFO) Resolution 118/2012 – Code of Dental Ethics (CEO) and Brazilian Law No. 5,081/1966 (L5081). The answer was “yes” in all cases, and when not provided, the question “What is the source of this information?” was asked. Answers were classified as “Correct,” “Partially correct,” and “Incorrect.” Errors were then reassessed with a reverse question: “What is described in Art. X of L5081?”. The same approach was applied to errors in the CEO, with classifications of “Correction,” “Same error,” and “New error.” Data were analyzed descriptively and using the Kruskal-Wallis test (α=5%). Results: Among answers, 134 (47.7%) were “Correct,” 127 (45.2%) were “Partially correct,” and 20 (7.1%) were “Incorrect.” Excluding the CEO’s initial and final provisions, Chapter XVII had the highest percentage of correct answers (n=7, 77.8%), while Chapter X had the highest rate of “Incorrect” answers (n=1, 25.0%). Of the 147 errors, 98.6% (n=145) generated a “New error.” No statistically significant difference was found among CEO chapters (p=0.058), but there was one between CEO and L5081 (p=0.005). Conclusion: ChatGPT®’s knowledge of Brazilian dental ethics and legislation may contain inaccuracies, and its use without source verification is not recommended.
2020-08-09
Sharma, Disha, Purayil, Tina Puthen, Ginjupalli, Kishore, Pentapati, Kalyana Chakravarthy
Sharma, Disha ; Purayil, Tina Puthen ; Ginjupalli, Kishore ; Pentapati, Kalyana Chakravarthy ; ABSTRACT Objective: To evaluate and compare the effects of 17% Ethylenediaminetetraacetic acid (EDTA) and 7% Maleic Acid (MA) on the push-out bond strength (POBS) and compressive strength (CS) of Biodentine and Bio-C Repair when used for furcal perforation repair. Material and Methods: Sixty extracted human mandibular molars were decoronated, and standardized furcal perforations were created. The teeth were mounted and randomly divided into two groups: Group I, Biodentine, and Group II, Bio-C Repair. Each group was irrigated with 2.5% Sodium Hypochlorite and then subdivided into three: Group A – 17% EDTA, Group B – 7% MA, and Group C – Distilled Water. For CS evaluation, eighteen cylindrical samples of each material were prepared, immersed in NaOCl, and similarly subdivided into three groups based on the irrigant used. All samples were tested using a universal testing machine. Results: Biodentine showed no significant difference in POBS among the irrigants (p=0.065). Bio-C Repair showed significantly higher POBS with MA and distilled water than with EDTA (p<0.001). Biodentine’s CS was significantly affected by the chelating agents (Control > EDTA > MA), whereas Bio-C Repair showed no significant difference; however, its CS remained lower than that of Biodentine. Conclusion: Chelating agents did not affect Biodentine's POBS, whereas MA improved Bio-C Repair's POBS over EDTA. Despite the reduction, Biodentine's CS remained higher than Bio-C Repair.
2020-08-09
Mishra, Swastik, Varghese, Jothi M., Kabekkodu, Shama Prasada
Mishra, Swastik ; Varghese, Jothi M. ; Kabekkodu, Shama Prasada ; ABSTRACT Objective: To explore the proliferative and wound-healing potential of Periostin and Emdogain® on periodontal fibroblasts. Material and Methods: Human PDL (hPDL) fibroblasts were cultured and treated with two biological agents, Emdogain® and Periostin. The Dulbecco's Modified Eagle Medium (DMEM) served as the control. The cytotoxic effects of Emdogain® and Periostin were analyzed using the Clonogenic assay. Cell viability was estimated using the CCK-8 assay. Further, the migratory potential of the hPDL fibroblasts was assessed using the scratch migration assay. Results: The results showed that 1/1000 dilution of Emdogain® and 1/10 dilution of Periostin had the highest cell survival fraction (p<0.05). In the CCK-8 assay, Emdogain® displayed maximum cell viability compared to Periostin (p=0.000) and control (p=0.006). The PDL fibroblasts' migratory rate corresponded to the experimental agents' wound-healing potential. Emdogain® presented with earlier wound closure compared to Periostin (p=0.002) and control (p=0.000). Conclusion: Thus, based on the results of this study, Periostin may be a viable biologic agent for promoting periodontal regeneration.
2020-08-09
Custodio, Isabela do Carmo, Souza, Bianca Katsumata de, Alcalde, Murilo Priori, Cruvinel, Thiago, Sakai, Vivien Thiemy, Lourenço Neto, Natalino
Custodio, Isabela Do Carmo ; Souza, Bianca Katsumata De ; Alcalde, Murilo Priori ; Cruvinel, Thiago ; Sakai, Vivien Thiemy ; Lourenço Neto, Natalino ; ABSTRACT Objective: To compare the adequate time, canal transportation, remaining resin quantity, and volume after instrumentation with different manual and mechanized files. Material and Methods: Eighty root canals from prototyped deciduous molars were divided into four groups according to instrumentation: WOG - reciprocating file; SMF - nickel-titanium manual file; K - manual stainless steel file; SBF - rotary file for deciduous teeth. Micro-CT scans were taken before and after instrumentation, and data were collected and statistically analyzed. Two-way ANOVA and Tukey's test were used for parametric results, and Kruskal-Wallis and Dunn's test for nonparametric results. Results: Mechanized groups showed shorter times than manual groups (p≤0.05). In canal transportation, the WOG group showed greater deviation than the other groups (p=0.002). The SBF group showed the highest remaining resin quantity (p=0.008). All groups showed statistically significant differences between apical and cervical thirds (p=0.008), although no differences in volume were observed among groups (p≥0.05). Conclusion: NiTi files (SMF) are a viable alternative for pulpectomy in deciduous teeth, preserving original canal anatomy without excessively altering root canal volume, providing efficient treatment in reduced time, whether used manually (SMF) or mechanized nickel-titanium files (WOG and SBF).